Application

Assurance^® GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance^® GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.

Compatibility

BioControl GDS

Components

Top STEC (eae) Amplification Tubes Tq Top 7 STEC Concentration Reagent Resuspension Buffer Tq Top STEC Wash Solution

Features and Benefits

Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance^® GDS Shiga Toxin Genes Top 7 assay. Top 7 STEC (includes E. coli O157:H7) Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

General description

Assurance^® GDS is an advanced genetic detection system for food pathogens. The Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following Top 7 O serogroups of E. coli: O103, O111, O121, O145, O26, O45, and O157. Assurance^® GDS utilizes a proprietary PickPen^® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the presence of the eae gene.

Legal Information

AOAC is a registered trademark of AOAC International

Assurance GDS is a registered trademark of BioControl Systems, Inc.

Assurance is a registered trademark of BioControl Systems, Inc.

PICKPEN is a registered trademark of BioControl Systems, Inc.

Testing Method

PCR, Immunomagnetic Separation (IMS)